Cover for A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction

Book2020

Author:

Jianghui Hou

A Laboratory Guide to the Tight Junction

Book2020

 

Cover for A Laboratory Guide to the Tight Junction

Author:

Jianghui Hou

About the book

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A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its ... read full description

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  2. Book chapterAbstract only

    Chapter 1 - Introduction

    Pages 1-32

  3. Book chapterAbstract only

    Chapter 2 - Biochemical approaches for tight junction

    Pages 33-88

  4. Book chapterAbstract only

    Chapter 3 - Biophysical approaches for tight junction

    Pages 89-151

  5. Book chapterAbstract only

    Chapter 4 - Histological approaches for tight junction

    Pages 153-174

  6. Book chapterAbstract only

    Chapter 5 - Light microscopy for tight junction

    Pages 175-211

  7. Book chapterAbstract only

    Chapter 6 - Electron microscopy for tight junction

    Pages 213-261

  8. Book chapterAbstract only

    Chapter 7 - Cell models of tight junction biology

    Pages 263-301

  9. Book chapterAbstract only

    Chapter 8 - Mouse models of tight junction physiology

    Pages 303-340

  10. Book chapterAbstract only

    Chapter 9 - Perspective

    Pages 341-377

  11. Book chapterNo access

    Index

    Pages 379-396

About the book

Description

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models.

Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research.

Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models.

Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research.

Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.

Key Features

  • Provides consistent and detailed research methods
  • Covers various cell, tissue and animal models
  • Includes step-by-step guidance from beginner to sophisticated levels
  • Provides consistent and detailed research methods
  • Covers various cell, tissue and animal models
  • Includes step-by-step guidance from beginner to sophisticated levels

Details

ISBN

978-0-12-818647-3

Language

English

Published

2020

Copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Imprint

Academic Press

Authors

Jianghui Hou

Division of Nephrology, Washington University Saint Louis, St. Louis, MO, United States