Cover for Laboratory Procedures and their Applications

Laboratory Procedures and their Applications

Book1984

Edited by:

INDRA K. VASIL

Laboratory Procedures and their Applications

Book1984

 

Cover for Laboratory Procedures and their Applications

Edited by:

INDRA K. VASIL

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  1. Full text access
  2. Book chapterNo access

    CHAPTER 1 - Organization of a Plant Tissue Culture Laboratory

    Daniel C.W. Brown and Trevor A. Thorpe

    Pages 1-12

  3. Book chapterNo access

    CHAPTER 2 - Educational Services for Plant Tissue Culture

    Paul J. Bottino

    Pages 13-17

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    CHAPTER 3 - Plant Cell Cultures: Nutrition and Media

    Oluf L. Gamborg

    Pages 18-26

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    CHAPTER 4 - Callus Culture: Induction and Maintenance

    F. Constabel

    Pages 27-35

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    CHAPTER 5 - Induction and Maintenance of Embryogenic Callus Cultures of Gramineae

    Vimla Vasil and Indra K. Vasil

    Pages 36-42

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    CHAPTER 6 - Clonal Propagation: Shoot Cultures

    Horst Binding and Gabriela Krumbiegel-Schroeren

    Pages 43-48

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    CHAPTER 7 - Clonal Propagation: Adventitious Buds

    Trevor A. Thorpe and Kamlesh R. Patel

    Pages 49-60

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    CHAPTER 8 - Clonal Propagation: Orchids

    Yoneo Sagawa and John T. Kunisaki

    Pages 61-67

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    CHAPTER 9 - Clonal Propagation: Somatic Embryos of Citrus

    T.S. Rangan

    Pages 68-73

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    CHAPTER 10 - Clonal Propagation: Palms

    Brent Tisserat

    Pages 74-81

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    CHAPTER 11 - Clonal Propagation: Gymnosperms

    Jenny Aitken-Christie and Trevor A. Thorpe

    Pages 82-95

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    CHAPTER 12 - Culture Methods for Bryophytes

    Martin Bopp and Bernd Knoop

    Pages 96-105

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    CHAPTER 13 - Culture of Shoot Meristems: Pea

    K.K. Kartha

    Pages 106-110

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    CHAPTER 14 - Culture of Shoot Meristems: Fruit Plants

    Olivia C. Broome and Richard H. Zimmerman

    Pages 111-122

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    CHAPTER 15 - The Acclimatization of Micropropagated Plants

    D.I. Dunstan and K.E. Turner

    Pages 123-129

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    CHAPTER 16 - Induction and Maintenance of Cell Suspension Cultures

    Patrick J. King

    Pages 130-138

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    CHAPTER 17 - Induction, Maintenance, and Manipulation of Development in Embryogenic Cell Suspension Cultures

    Philip V. Ammirato

    Pages 139-151

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    CHAPTER 18 - Isolation and Maintenance of Embryogenic Cell Suspension Cultures of Gramineae

    Vimla Vasil and Indra K. Vasil

    Pages 152-158

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    CHAPTER 19 - Fractionation of Cultured Cells

    Tatsuhito Fujimura and Atsushi Komamine

    Pages 159-166

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    CHAPTER 20 - Large-Scale Cultures of Cells in Suspension

    M.W. Fowler

    Pages 167-174

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    CHAPTER 21 - Synchronization of Suspension Culture Cells

    A.S. Wang and R.L. Phillips

    Pages 175-181

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    CHAPTER 22 - Photoautotrophic Cell Cultures

    Wolfgang Hüsemann

    Pages 182-191

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    CHAPTER 23 - Quantitative Plating Technique

    Robert B. Horsch

    Pages 192-198

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    CHAPTER 24 - The Feeder Layer Technique

    D. Aviv and E. Galun

    Pages 199-203

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    CHAPTER 25 - Culture of Isolated Mesophyll Cells

    Hans Willy Kohlenbach

    Pages 204-212

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    CHAPTER 26 - The Multiple-Drop-Array (MDA) Screening Technique

    Christian T. Harms

    Pages 213-220

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    CHAPTER 27 - Culture of Ovaries

    T.S. Rangan

    Pages 221-226

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    CHAPTER 28 - Culture of Ovules

    T.S. Rangan

    Pages 227-231

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    CHAPTER 29 - Culture of Cotton Ovules

    C.A. Beasley

    Pages 232-240

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    CHAPTER 30 - Culture of Embryos

    G.B. Collins and J.W. Grosser

    Pages 241-257

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    CHAPTER 31 - Culture of Endosperm

    Sant S. Bhojwani

    Pages 258-268

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    CHAPTER 32 - In Vitro Pollination and Fertilization

    Maciej Zenkteler

    Pages 269-275

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    CHAPTER 33 - In Vitro Pollination, Fertilization, and Development of Maize Kernels

    Burle G. Gengenbach

    Pages 276-282

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    CHAPTER 34 - Anther Culture of Nicotiana tabacum

    N. Sunderland

    Pages 283-292

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    CHAPTER 35 - Anther Culture of Solanum tuberosum

    G. Wenzel and B. Foroughi-Wehr

    Pages 293-301

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    CHAPTER 36 - Anther Culture of Brassica

    W.A. Keller

    Pages 302-310

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    CHAPTER 37 - Anther Culture of Cereals and Grasses

    G. Wenzel and B. Foroughi-Wehr

    Pages 311-327

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    CHAPTER 38 - Isolation and Culture of Protoplasts: Tobacco

    Itaru Takebe and Toshiyuki Nagata

    Pages 328-339

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    CHAPTER 39 - Isolation and Culture of Protoplasts: Petunia

    Horst Binding and Gabriela Krumbiegel-Schroeren

    Pages 340-349

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    CHAPTER 40 - Isolation and Culture of Protoplasts: Datura

    O. Schieder

    Pages 350-355

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    CHAPTER 41 - Isolation and Culture of Protoplasts: Brassica

    Hellmut R. Schenck and Franz Hoffmann

    Pages 356-369

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    CHAPTER 42 - Isolation and Culture of Protoplasts: Tomato

    Elias A. Shahin

    Pages 370-380

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    CHAPTER 43 - Isolation, Culture, and Regeneration of Potato Leaf Protoplasts from Plants Preconditioned in Vitro

    Elias A. Shahin

    Pages 381-390

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    CHAPTER 44 - Isolation and Culture of Protoplasts from Carrot Cell Suspension Cultures

    Denes Dudits

    Pages 391-397

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    CHAPTER 45 - Isolation and Culture of Embryogenic Protoplasts of Cereals and Grasses

    Vimla Vasil and Indra K. Vasil

    Pages 398-404

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    CHAPTER 46 - Mechanical Isolation and Single-Cell Culture of Isolated Protoplasts and Somatic Hybrid Cells

    Y.Y. Gleba, V.A. Sidorov and Franz Hoffmann

    Pages 405-413

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    CHAPTER 47 - Fusion of Protoplasts by Polyethylene Glycol (PEG)

    F. Constabel

    Pages 414-422

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    CHAPTER 48 - Fusion of Protoplasts by Dextran and Electrical Stimulus

    Toshiaki Kameya

    Pages 423-427

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    CHAPTER 49 - Inactivation of Protoplasts before Fusion to Facilitate Selective Recovery of Fusion-Derived Clones

    Laszlo Menczel

    Pages 428-432

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    CHAPTER 50 - Selection of Somatic Hybrid Cells by Fluorescence-Activated Cell Sorting

    David W. Galbraith

    Pages 433-447

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    CHAPTER 51 - Enucleation of Protoplasts: Preparation of Cytoplasts and Miniprotoplasts

    Horst Lörz

    Pages 448-453

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    CHAPTER 52 - Isolation of Organelles: Nuclei

    L. Willmitzer

    Pages 454-460

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    CHAPTER 53 - Isolation of Organelles: Chromosomes

    Gyula Hadlaczky

    Pages 461-470

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    CHAPTER 54 - Isolation of Organelles: Chloroplasts

    J. Kobza and G.E. Edwards

    Pages 471-482

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    CHAPTER 55 - Liposome Preparation and Incubation with Plant Protoplasts

    Robert T. Fraley

    Pages 483-491

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    CHAPTER 56 - Inoculation of Protoplasts with Plant Viruses

    Itaru Takebe

    Pages 492-502

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    CHAPTER 57 - Uptake of Organelles

    Anita Wallin

    Pages 503-513

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    CHAPTER 58 - Transformation of Tobacco Cells by Coculture with Agrobacterium tumefaciens

    László Márton

    Pages 514-521

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    CHAPTER 59 - Ti-Plasmid DNA Uptake and Expression by Protoplasts of Nicotiana tabacum

    F.A. Krens and R.A. Schilperoort

    Pages 522-534

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    CHAPTER 60 - Immobilization of Cultured Plant Cells and Protoplasts

    P. Brodelius

    Pages 535-546

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    CHAPTER 61 - Mutagenesis of Cultured Cells

    Patrick J. King

    Pages 547-551

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    CHAPTER 62 - Cell Culture Procedures for Mutant Selection and Characterization in Nicotiana plumbaginifolia

    Pal Maliga

    Pages 552-562

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    CHAPTER 63 - Induction, Selection, and Characterization of Mutants in Carrot Cell Cultures

    J.M. Widholm

    Pages 563-570

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    CHAPTER 64 - Induction, Selection, and Characterization of Mutants in Maize Cell Cultures

    Kenneth A. Hibberd

    Pages 571-576

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    CHAPTER 65 - Elimination of Viruses

    K.K. Kartha

    Pages 577-585

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    CHAPTER 66 - Cocultures of Plant and Bacterial Cells

    Minocher Reporter

    Pages 586-597

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    CHAPTER 67 - Isolation and Bioassay of Fungal Phytotoxins

    Jonathan D. Walton and Elizabeth D. Earle

    Pages 598-607

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    CHAPTER 68 - Freeze Preservation of Cells

    Lyndsey A. Withers

    Pages 608-620

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    CHAPTER 69 - Freeze Preservation of Meristems

    K.K. Kartha

    Pages 621-628

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    CHAPTER 70 - Selection of Cell Lines for High Yields of Secondary Metabolites

    Yasuyuki Yamada

    Pages 629-636

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    CHAPTER 71 - Isolation and Analysis of Terpenoids

    Joseph H. Lui

    Pages 637-643

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    CHAPTER 72 - Isolation and Analysis of Alkaloids

    W.G.W. Kurz

    Pages 644-650

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    CHAPTER 73 - Protein Extraction and Analysis

    L.R. Wetter

    Pages 651-658

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    CHAPTER 74 - Isolation and Analysis of Plant Growth Regulators

    Kerry T. Hubick and David M. Reid

    Pages 659-683

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    CHAPTER 75 - Plastic Embedding for Light Microscopy

    Claudia Botti and Indra K. Vasil

    Pages 684-688

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    CHAPTER 76 - Histological and Histochemical Staining Procedures

    Edward C. Yeung

    Pages 689-697

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    CHAPTER 77 - Staining and Nuclear Cytology of Cultured Cells

    Alan R. Gould

    Pages 698-711

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    CHAPTER 78 - Chromosome Analysis

    R.L. Phillips and A.S. Wang

    Pages 712-727

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    CHAPTER 79 - Preparation of Cultured Cells and Tissues for Transmission Electron Microscopy

    Larry C. Fowke

    Pages 728-737

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    CHAPTER 80 - Preparation of Cultured Tissues for Scanning Electron Microscopy

    Vimla Vasil and Indra K. Vasil

    Pages 738-743

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    CHAPTER 81 - Microspectrophotometric Analysis

    Jerome P. Miksche and Sukhraj S. Dhillon

    Pages 744-752

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    CHAPTER 82 - Cell Cycle Analysis by Conventional Methods

    Alan R. Gould

    Pages 753-764

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    CHAPTER 83 - Flow Cytometric Analysis of the Cell Cycle

    David W. Galbraith

    Pages 765-777

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    CHAPTER 84 - Autoradiography

    Edward C. Yeung

    Pages 778-784

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    CHAPTER 85 - Immunofluorescence Techniques for Studies of Plant Microtubules

    Larry C. Fowke, Daina Simmonds, ... Pieter van der Valk

    Pages 785-794

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    Index

    Pages 795-825

About the book

Details

ISBN

978-0-12-715001-7

Language

English

Published

1984

Copyright

Copyright © 1984 Elsevier Inc. All rights reserved.

Imprint

Academic Press

Editors

INDRA K. VASIL

Department of Botany, University of Florida, Gainesville, Florida